Regulatory

Part:BBa_K1955003:Design

Designed by: Justine Hsu   Group: iGEM16_CGU_Taiwan   (2016-10-14)


Leishmania-5 prime UTR-Hygromycin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The hemagglutinin (HA) part is a 1700 bp DNA sequence acquired from GenBank (Accession# J02176.1). Some nucleotides of HA sequence had been changed to avoid the restriction sites. In order to compare the protein expression efficiency of our expression vector and the original pX63HYG shuttle vector, we add BamHI restriction site at the head and tail of HA coding sequence for the ligation of pX63HYG shuttle vector and HA sequence.


Source

The whole sequence of Hemagglutinin (HA) from H1N1 is acquired from GenBank.

References